Question 1

All of the following are biochemical tests for the identification of Mycobacterium spp., except

Accepted Answer

A)   gelatin liquefaction.
B)   hydrolysis of Tween 80.
C)   iron uptake.
D)   arylsulfatase.E) A) and C)
F) C) and D)

Question 2

All of these stains are commonly used to visualize mycobacteria on a smear, except

Accepted Answer

A)   Ziehl-Neelsen.
B)   Kinyoun.
C)   auramine.
D)   acridine orange.E) B) and D)
F) None of the above

Question 3

What is the single most important piece of equipment in a mycobacterial laboratory?

Accepted Answer

A)   Ultraviolet lights
B)   Covered centrifuge
C)   Biological safety cabinet
D)   CO2 incubatorE) A) and B)
F) A) and C)

Question 4

A sputum culture from a patient in Texas has buff colored colonies growing in approximately 4 weeks. After exposure to light, the colonies turn intense yellow. This isolate is most likely

Accepted Answer

A)   M. scrofulaceum.
B)   M. kansasii.
C)   M. fortuitum.
D)   M. xenopi.E) A) and B)
F) B) and C) B

Question 5

A child presents to his physician with fever, a nonproductive cough, and shortness of breath. The physician orders a routine sputum culture and an acid-fast bacillus culture and smear. The smear shows red organisms arranged in ropes. What is the most probable cause of this child's fever and cough?

Accepted Answer

A)   Mycobacterium leprae
B)   M. avium
C)   M. intracellulare
D)   M. tuberculosisE) A) and B)
F) All of the above

Question 6

All of the following are traditional characteristics used to identify mycobacteria, except

Accepted Answer

A)   rate of growth.
B)   colony morphology.
C)   Gram stain results.
D)   pigmentation.E) All of the above
F) B) and D) C

Question 7

What is the purpose of the digestion-decontamination processing of specimens submitted for mycobacterial culture?

Accepted Answer

A)   To release all bacteria in the sample
B)   To make the specimen easier to plate
C)   To allow chemical decontaminants to kill nonmycobacterial organisms
D)   To liquefy the outer cell wall of the mycobacteria so that they will grow fasterE) B) and C)
F) None of the above

Question 8

What media are recommended for routine culturing of specimens for the recovery of acid-fast bacilli?

Accepted Answer

A)   Löwenstein-Jensen (LJ)  and liquid-based media
B)   Middlebrook 7H10 and cooked meat broth
C)   Sabouraud dextrose and LIM broth
D)   Thiosulfate-citrate-bile salts-sucrose (TCBS)  and thioglycollate brothE) None of the above
F) A) and C)

Question 9

What medium is recommended for the recovery of Mycobacterium haemophilum?

Accepted Answer

A)   Sheep blood agar (SB
A)  
B)   Chocolate
C)   Polysaccharide egg antigen (PE
A)  
D)   LJE) None of the above
F) B) and C)

Question 10

A 35-year-old man travels on a multidestination vacation over a 2 month period, including lengthy stops in tropical Africa and Southeast Asia. Six weeks after returning come home to the United States, he develops a productive cough, fatigue, weight loss, low-grade fever, and night sweats. What disease should the physician consider as a result of his travel history?

Accepted Answer

A)   Streptococcal pneumonia
B)   Primary atypical pneumonia
C)   TB
D)   Pneumonia caused by gram-negative rodsE) A) and B)
F) B) and C)

Question 11

What are nonchromogens?

Accepted Answer

A)   Species that produce carotene pigment upon exposure to light
B)   Species that produce pigment in the light or the dark
C)   Species whose colonies remain buff colored after exposure to light
D)   Species whose colonies fluoresce under ultraviolet lightE) B) and C)
F) A) and B)

Question 12

What component of mycobacteria is used for identification in nucleic acid hybridization assays?

Accepted Answer

A)   DNA
B)   Ribosomal RNA
C)   Transfer RNA
D)   Messenger RNAE) A) and B)
F) A) and D)

Question 13

What classifies an organism as a multidrug-resistant Mycobacterium tuberculosis?

Accepted Answer

A)   Resistance to at least isoniazid and rifampin
B)   Resistance to isoniazid, rifampin, ethambutol, and streptomycin
C)   Resistance to streptomycin and rifampin
D)   Resistance to ciprofloxacin and imipenemE) A) and B)
F) None of the above A

Question 14

The most common nontuberculosis mycobacterium (NTM)  infecting humans

Accepted Answer

A)   M. marinum.
B)   M. ulcerans.
C)   M. haemophilium.
D)   M. avium-intracellulare complex.E) A) and B)
F) B) and C)

Question 15

What constituent of LJ medium is added to suppress the growth of gram-positive bacteria?

Accepted Answer

A)   Carbolfuchsin
B)   Malachite green
C)   Gentian purple
D)   MercurochromeE) All of the above
F) None of the above

Question 16

What are scotochromogens?

Accepted Answer

A)   Species that produce carotene pigment upon exposure to light
B)   Species that produce pigment in the light or the dark
C)   Species whose colonies remain buff colored after exposure to light
D)   Species whose colonies fluoresce under ultraviolet lightE) A) and B)
F) A) and C)

Question 17

Middlebrook 7H10 and 7H11 media are enriched with all of the following, except

Accepted Answer

A)   oleic acid.
B)   bovine albumin.
C)   heme.
D)   beef catalase.E) B) and C)
F) A) and B)

Question 18

If a person has had TB during his or her lifetime, how likely is it that the person will get the disease again?

Accepted Answer

A)   5% to 15%
B)   20% to 25%
C)   1%
D)   50%E) A) and D)
F) B) and C)

Question 19

The Centers for Disease Control and Prevention (CDC)  recommends all the following drugs for the treatment of Mycobacterium tuberculosis, except

Accepted Answer

A)   ethambutol.
B)   isoniazid.
C)   rifampin.
D)   ciprofloxacin.E) B) and C)
F) A) and D)

Question 20

What is the optimum culture incubation time for most mycobacteria associated with human disease?

Accepted Answer

A)   2 to 6 weeks
B)   1 to 2 weeks
C)   5 to 7 days
D)   3 to 5 daysE) A) and B)
F) A) and C)

Exam 26: Mycobacterium Tuberculosis and Nontuberculous Mycobacteria

All of the following are biochemical tests for the identification of Mycobacterium spp., except

Correct Answer
verified

All of these stains are commonly used to visualize mycobacteria on a smear, except

Correct Answer
verified

What is the single most important piece of equipment in a mycobacterial laboratory?

Correct Answer
verified

A sputum culture from a patient in Texas has buff colored colonies growing in approximately 4 weeks. After exposure to light, the colonies turn intense yellow. This isolate is most likely

Correct Answer
verified
B

A child presents to his physician with fever, a nonproductive cough, and shortness of breath. The physician orders a routine sputum culture and an acid-fast bacillus culture and smear. The smear shows red organisms arranged in ropes. What is the most probable cause of this child's fever and cough?

Correct Answer
verified

All of the following are traditional characteristics used to identify mycobacteria, except

Correct Answer
verified
C

What is the purpose of the digestion-decontamination processing of specimens submitted for mycobacterial culture?

Correct Answer
verified

What media are recommended for routine culturing of specimens for the recovery of acid-fast bacilli?

Correct Answer
verified

What medium is recommended for the recovery of Mycobacterium haemophilum?

Correct Answer
verified

Correct Answer
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What are nonchromogens?

Correct Answer
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What component of mycobacteria is used for identification in nucleic acid hybridization assays?

Correct Answer
verified

What classifies an organism as a multidrug-resistant Mycobacterium tuberculosis?

Correct Answer
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A

The most common nontuberculosis mycobacterium (NTM) infecting humans

Correct Answer
verified

What constituent of LJ medium is added to suppress the growth of gram-positive bacteria?

Correct Answer
verified

What are scotochromogens?

Correct Answer
verified

Middlebrook 7H10 and 7H11 media are enriched with all of the following, except

Correct Answer
verified

If a person has had TB during his or her lifetime, how likely is it that the person will get the disease again?

Correct Answer
verified

The Centers for Disease Control and Prevention (CDC) recommends all the following drugs for the treatment of Mycobacterium tuberculosis, except

Correct Answer
verified

What is the optimum culture incubation time for most mycobacteria associated with human disease?

Correct Answer
verified

Exam 26: Mycobacterium Tuberculosis and Nontuberculous Mycobacteria

All of the following are biochemical tests for the identification of Mycobacterium spp., except

Correct AnswerverifiedShow Answer

All of these stains are commonly used to visualize mycobacteria on a smear, except

Correct AnswerverifiedShow Answer

What is the single most important piece of equipment in a mycobacterial laboratory?

Correct AnswerverifiedShow Answer

A sputum culture from a patient in Texas has buff colored colonies growing in approximately 4 weeks. After exposure to light, the colonies turn intense yellow. This isolate is most likely

Correct AnswerverifiedB

A child presents to his physician with fever, a nonproductive cough, and shortness of breath. The physician orders a routine sputum culture and an acid-fast bacillus culture and smear. The smear shows red organisms arranged in ropes. What is the most probable cause of this child's fever and cough?

Correct AnswerverifiedShow Answer

All of the following are traditional characteristics used to identify mycobacteria, except

Correct AnswerverifiedC

What is the purpose of the digestion-decontamination processing of specimens submitted for mycobacterial culture?

Correct AnswerverifiedShow Answer

What media are recommended for routine culturing of specimens for the recovery of acid-fast bacilli?

Correct AnswerverifiedShow Answer

What medium is recommended for the recovery of Mycobacterium haemophilum?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

What are nonchromogens?

Correct AnswerverifiedShow Answer

What component of mycobacteria is used for identification in nucleic acid hybridization assays?

Correct AnswerverifiedShow Answer

What classifies an organism as a multidrug-resistant Mycobacterium tuberculosis?

Correct AnswerverifiedA

The most common nontuberculosis mycobacterium (NTM) infecting humans

Correct AnswerverifiedShow Answer

What constituent of LJ medium is added to suppress the growth of gram-positive bacteria?

Correct AnswerverifiedShow Answer

What are scotochromogens?

Correct AnswerverifiedShow Answer

Middlebrook 7H10 and 7H11 media are enriched with all of the following, except

Correct AnswerverifiedShow Answer

If a person has had TB during his or her lifetime, how likely is it that the person will get the disease again?

Correct AnswerverifiedShow Answer

The Centers for Disease Control and Prevention (CDC) recommends all the following drugs for the treatment of Mycobacterium tuberculosis, except

Correct AnswerverifiedShow Answer

What is the optimum culture incubation time for most mycobacteria associated with human disease?

Correct AnswerverifiedShow Answer

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B

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C

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A

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